Reporter

Part:BBa_K4799608:Design

Designed by: Wanjun Chen   Group: iGEM23_BGI-MammothEdu-China   (2023-10-10)


pAtPCR2_GUS_T-Nos


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 22
    Illegal XbaI site found at 1249
    Illegal SpeI site found at 1572
    Illegal SpeI site found at 2043
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 22
    Illegal NheI site found at 3852
    Illegal SpeI site found at 1572
    Illegal SpeI site found at 2043
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 22
    Illegal BglII site found at 1834
    Illegal BamHI site found at 1817
    Illegal XhoI site found at 477
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 22
    Illegal XbaI site found at 1249
    Illegal SpeI site found at 1572
    Illegal SpeI site found at 2043
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 22
    Illegal XbaI site found at 1249
    Illegal SpeI site found at 1572
    Illegal SpeI site found at 2043
    Illegal NgoMIV site found at 2267
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2067
    Illegal BsaI.rc site found at 2682
    Illegal SapI.rc site found at 2853


Design Notes

Test if the promoter will induce GUS express after cadmium treatment.



Source

plasmid

References